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Comparative Studies of Organic Inorganic and Normal Growth of Ocimum Ttanuiflorum and Ocimum Gratissimum by Extraction of UV and HPLC.

Abstract

The foliage of Tilsi has a strong smell,green or purple in color and taste similar to clove. It grows wild in the tropics and warm regions. It has great value in the Hindu culture. Tulsi has great potential owing to its valuable Cosmetic properties.Its essence is used in various forms like face-washes, power, pulp,herbal soap, oil etc. In ayurveda , Tulsi is known as ‘’Queen of Herbs’’.The top fifteen medicinal uses of Tulsi are as follows: Healing Power, Fever and common cold,Coughs, Respiratory Disorder, Kideny Stone, Stress, Mouth Infections, Insect Bites, Teeth Disorder, Headaches, Eye Disorders.

The experimenral material consist of two species: Ocimum Gratissimum’(Ram tulsi) and Ocimum tenuiflorum’(Krishna tulsi) plant which are grown in three composition organic, Inorganic,and in Normal fertilizer.

These plants are grown in varios fertilizer:-

Organic fertilizer :- Urea,Cow -dung, Humic acid

Inorganic fertilizer:-Na,P,K

Normal Fertilizer :- Black Soil.

Chemical Reagent used Ethylene Diamine tetra acetate(EDTA)

Amit Upadhyay

PG Tech Research Institute

SYNTHESIS, CHARACTERIZATION AND ANTIBACTERIAL ACTIVITY OF N SULPHONAMIDE DRUG AND ANALOGUE OF SULPHONAMIDE DRUG

Abstract

The science dealing with the study of prevention and treatment of diseases caused by micro-organisms is known as medical microbiology. The different disciplines of microbiology are studied under following heads,as follows: virology (study of viruses), Bacteriology(study of bacteria),mycology(study of fungi) phycology (study of algae) and protozoology (study of protozoa) .To kill these organism different types of chemicals are used and these chemicals agents are called the antimicrobial agents.

In this research we are focusing on the executed plan of work which invoilves proper selection of drugs, theire synthesis and characterization, selection and optimization of suitable reactions for the synthesis of mutual drugs, characterization of the synthesized products, and in vitro studies of the synthesized drugs. Each of these areas is studied in detail for the fruitful research outcome.

The objective taken in this research are as follows:

  • Synthesis of structurally analogue of sulphonamide drug.
  • Comparative study of drugs with sulphonamides using Fourier transform infrared spectroscopy(FTIR)
  • Comparative study of synthesized drug as antibactetial with reference to the sulphonamide drugs.
  • In future, the research of more antibacterial drugs with their effect and more active against bacteria.

Poornima Paliwal

PG Tech Research Institute 

FORMULATION AND CHARECTRISTIC OF BILAYER TABLET OF ANTIRETROVIRAL DRUG(ABACAVIR/LAMIVUDINE)

Abstract

HIV, the causative agent of AIDS, is a single-stranded RNA that belong to the family of Retroviridae. Its genetic information is not encoded as DNA, but as RNA and therefore is reserve-transcribed into DNA. HIV is round partical with a size of between 100-120nm in diameter and it is surround by a lipid bilayer. It has half-life of less than 2 hours.

Tablet may be defined as solid pharmaceutical dosage forms containing drug substance with or without suitable diluents and prepared either by compression or molding methods.The tablet must be sufficiently strong and resistance to shock and abrasion and to without handling, during manufacturing, packing, shipping, and use. Hardness and friability tests measure this property.

Plan of Work

  1. Review of Literature.
  2. Preformulation study of drugs and excipients.
  3. Precompretion parameter
  4. Drug excipient compatibility study
  5. Macroscopic properties
  6. Microscopic properties
  7. IR Spactroscopy
  8. Preparation of stable API-1,API-2 bilayer tablet.
  9. Optimization of concentration of excipients and method of manufacturing
  10. Evaluation of physical parameter
  11. Like thickness
  12. Hardness
  13. Friability and
  14. Disintegration Time of tablets.
  15. Evaluation of In-vitro drug release of the tablets
  16. Selection of best formulation on the basis of In vitro drug release

                                                                                                                     Durga Patel

                                                                                                                  PG Tech Research Institute

Development and characterization of effective topical nanogel for corneal fungal infection

Abstract

Fungal infection of the eye are less common than infections with bacteria or viruses, but are usually severe and may lead to loss of vision.Infections may involve the cornea(Keratitis), the interior of the eye(endophthalmitis), or orbit, and may occur following trauma or systemic disseminated infection.According to the World Health Organization,corneal diseases are a major cause of vision loss and blindness.The term fungal keratitis refers to a corneal infection caused by fungi. One type of fungus that can infect the cornea is Fusarium. When Fusarium infects the cornea, the eye disease is referred to as Fusarium keratitis.

Plan of work:

The research work will be carried out under the following set of guidelines

  1. Literature survey
  2. Procurement of drug and polymer
  3. Identification of drugChemical tests
  • UV(λmax estimation)
  • IR (IR spectra of the sample)
  1. Preformulation studies
  • Physical characteristics
  • Melting point
  • Solubility studies
  • Partition coefficient studies
  • Preparation of standard curve
  1. Designing of carrier system
  • Preparation and optimization of nanogel
  1. Characterization of carrier system
  • Morphology
  • Size and Size distribution
  • Drug entrapment efficient
  • Surface charge
  1. In vitro drug release
  2. Stability studies
  3. Antifungal study
  4. In-vivo study

                  Neha Jha

PG Tech Research Institute

ANTIMICROBIAL ACTIVITY OF SOME SELECTED POISONOUS PLANT IN INDORE

Abstract

Poison a substance that, on ingestion, inhalation, absorption, application, injection, or development within the body, in relatively small amount, may cause structural damage or functional disturbance or A substance that ,when introduced into or absorbed by a living organism, causes death or injury.They are a different group from plant which cause illness if eaten in very large amount or have physical qualities that cause illness,e.g.clover in bloat, tree loppings in omasal impaction.The magnitude of animal losses to ingestion of poisonous plant .Losses include not only those due to mortality but also those due to poor productivity such as decreased weight gain or decreased milk or egg production.

Plan of work

The work envisaged is planned to proceed on the following lines

  1. Literature survey and procurement of material
  2. Material and method
  3. Collection of plant Material
  4. Solvent Extraction
  5. Growth and Maintenance of Test Microorganism for Antimicrobial studies
  6. Preparation of Inoculum
  7. Anti – bacterial activity
  8. Antifungal activity
  9. In – Vitro studies
  10. Data compilation and interpretation, result and discussion, summary and conclusion

Nitu Singh

PG Tech Research Institute

PHENOLIC COMPOUNDS CHARACTERIZATION AND BIOLOGYCAL ACTIVITIES OF CITRUS AURANTIUM BLOOM

Abstract

Citrus plants are known to possess beneficial activities for human health. In addition, ethnopharmacological application of plants is a good tool to explore their bioactivities and active compounds. This research was carried out to evaluate the phenolic and flavonoid analysis, antioxidant properties, anti cancer activities of Citrus aurantium bloom. The total phenolics and flavonoids compared to ethanolic and boiling waters extracts. The total obtained total phenolic values for methanolic Citrus aurantium bloom extracts was 4.55+_ 0.05mg gallic acid equivalent (GAE)/g dry weight (DW) and for total flavonoids it was 3.83 +0.05 mg rutin equivalent/g DW. In addition, the RP-HPLC of phenolics and flavonoids indicated the presence of gallic acid,pyrogallol,syringic acid ,caffein acid rutien ,quercetin and naringin as bioactive compounds. The antioxidant activities of citrus aurantium bloom were examined by the 1,1-diphenyl1-2 picryl-hydrazyl(DPPH) assay and the ferric reducing/ antioxidant potential(FRAP). The free radical scavenging and ferric reducing power activities were higher for the methanolic extract of citrus aurantium bloom at a concentration of 300 ug/ml,with value of 55.3% and 51.7%,respectively , as compared to the corresponding boiling water and ethanolic , but the activities were lower than those of antioxidant standards such as BHT and α-tocopherol.  Further more, the anti-inflammatory result of methanolic extract showed appreciable reduction in nitric oxide production of stimulated RAW 264.7 cell at the presence of plant extract. Apart  form that the anticancer activity of the methanolic extract was investigated in vitro against human cancer cell lines (MCF-7;MDA-MB-231),human colon adenocarcinoma (HT-29) and change cell as a normal human hepatocyte. The obtained result demonstrated the moderate to appreciable activities against all cell line tested and the compound present in the extract are non-toxic which make them suitable as potential therapeutics.

                                                                       prepared by

                                                 Shabaz Khan

Coordination with PGTRI

COMPARATIVE STUDY ON METHOD DEVELOPMENT AND VALIDATION OF LAMOTRIGINE IN TABLE DOSAGE FORM

ABSTRACT

A simple, rapid and precise method was developed for the quantitative

determination of Lamotrigine in tablet dosage from. The method was based on

RP-HPLC. Chromatographic separation was performed on a fortis C18 (150mm X

4.6mm and equivalent 5μm) column using a mobile phase of acetonitrile (ACN)

and phosphate buffer (0.05mol) ratio 20:80 at pH 2.5 adjusted with dilute

orthophosphoric acid. The following system conditions were maintained

throughout development and validation i.e., flow rate of mobile phase

1ml/min, column temperature at 40˚C and the detecting of drug by a UV-wave

length at 270 nm. The Lamotrigine was well resolved on the stationary phase and

the retention time was 5.1 minute. The method was validated the drug was

shown to be linear for drug in 10-30 μg /ml. the correlation coefficient (r2) for

the drug was 0.998. The Precision, Accuracy, LOD and LOQ were determined to

validate the method.

 

 

PREPARATION AND EVALUATION OF BILAYER TABLET OF FUROSIMIDE.

Abstract

For the purpose of enhancement the bioavailability of furosemide (FR), a floating dosage from with controlled release of FR was designed in this study.Because of the lower solubility of active material in the gastric medium , it was first enhanced by preparing an inclusion complex of FR with beta-cyclodextrin in a 1:1 proportion using the kneading method. Following the design of dosage from, bilayer floating tablets were prepared. After dissolution rate studies were performed using the continuous flow-through cell method, the formulation that provided delivery of active material near the target profile was given to six health male volunteer subjects,and in vivo tests were performed. Bilayered drugs delivery system is well known modified release dosage from may offer one or more advantages over immediate release formulations of the same drug.Also the formulation is much eaiser than the other controlled drugs delivery systems.The major aim of controlled drug delivery is to reduce the frequency of dosing.

Done By

Mr.Ramakant Sharma

from Satya Sai College of Pharmacy Indore

Co-ordination with 

PG Tech Research Institute

FORMULATION AND EVALUATION OF LIPOSOMES OF LAMIVUDINE

Abstract

Human Immunodeficiency Virus (HIV) Infection And Acquired Immune Deficiency Syndrome(AIDS) Commonly Referred To As HIV & AIDS Have Emerged As Being Amongst The Serious And Challenging Public Health Problems In the World.

Liposome are bilayer vesicles which have found use, among other applications, as drugs delivery vehicles.Conventional techniques for liposome preparation and size reduction remain popular as these are simple to implement and do not require sophisticathed equipment.However,issues related to scale-up for industrial production and scale-down for point-of-care applications have motivated improvements to conventional processes and have also led to the development of novel routes to liposome formation. In this article,these modified and new method for liposome preparation have been reviewed and classified with the objective of updating the reader to recent development in liposome production technology.

Synonyms:

4-amino-1-[(2R,5S)-2(hydroxymethyl)-1,3-oxathiolan-5-yl]-1,2-dihydropyrimidin-2-one

Prepared by

Ms Madhuri Meghwanshi 

Under PG Tech Research Institute

ADVANCE FOOD ANALYSIS AND QUALITY CONTROL

Abstract

For a health body, a balanced diet is required which is obtained from good nutrition through food products. These days, many people malpractice the adulteration of food products to gain profit in an illegal ways and earn money by deceiving the consumers. But, the adulteration of food is an emerging problem of concern which affects human health directly. There are a bundle of food adulterants which pose harmful effects to human, hence it is necessary for mankind to overcome this malpractice. In the present review , we will discuss about some of the food adulteterants causing serious health concerns to human beings.

                                                                                  Done By

Anjali Gupta

From Career College Bhopal

Coordination with

PG Tech Research Institute

DEVELOPMENT AND EVALUATION OF TOPICAL GEL OF VALTRX (VALACYCLOVIR) DRUG

ABSTRACT
Topical gels of valdecoxib topical gel prepared using different gelling agents(Viz,carbopol, HPMC , Sodium alginate, sodium CMC). Formulation were evaluated for pH, reheological behavior, drugs content and in vitro drug diffusion. Selected formulations of all the gelling agents appeared to be non-Newtonian and pseudo plastic behavior. Drug content was high(>98%) in gels.Drug release from the carbopol gels increased with the increase in the concentration of 20%. The drugs release increased with the increase in concentration of ethanol. In case of gels containing HPMC, sodium alginate, sodium CMC as gelling agents ,addition of PG up to 5%, increased the release of drug from the gels. However, release decreased with increase in the concentration of PG up to 10%. In case of HPMC GELS,ADDITION OF ETHANIL DECREASED THE RELEASE OF Valdecoxib from the gels. It is concluded that PG is a good penetration enhancer and carbopol good gelling agents for Valdecoxib gels.

Prepared By

Ms Aprana Joshi

under PG Tech Research Institute